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National Institutes of Health |
Notice: As of December 23, 2011, all NCRR programs have been transferred to other NIH Institutes and Centers.
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Rodent ResourcesON THIS PAGE:
Knockout Mouse Project (KOMP) RepositoryThe Knockout Mouse Project (KOMP) Repository was established in May 2007 to archive and distribute the approximately 8,500-10,000 KOMP vectors and ES cell clones) produced by the 2 KOMP Mutagenesis Teams at the CSD Consortium (Children's Hospital Oakland Research Institute [CHORI], The Sanger Institute, and UC Davis) and at Regeneron, Inc. The KOMP Repository is located at UC Davis and a subcontract to CHORI. The purpose of the KOMP Repository is to ensure the preservation, protection, availability, and accessibility of KOMP products, resources, and services for use by the scientific community. The repository is built upon the closely linked infrastructure, technical support, and faculty expertise within the UC Davis Mouse Biology Program (MBP), Center for Comparative Medicine, and Schools of Medicine and Veterinary Medicine, and the CHORI-BACPAC Resource. UC Davis shall archive, maintain, and distribute all KOMP ES cell clones, live mouse lines, and frozen embryos and sperm, while CHORI shall archive, maintain, and distribute all KOMP vectors. Research Emphasis/ObjectivesThe objectives of the KOMP Repository are to: 1) conduct QC procedures to verify the viability, identity, and utility of all KOMP products; 2) enable fast, simple, and easy online searching and ordering for any KOMP product or service (e.g., microinjection, ICSI for frozen B6 sperm, pathology analysis, etc); 3) distribute KOMP products promptly and for reasonable cost, and 4) provide responsive and attentive customer and technical service. These objectives will be accomplished by expanding, genotyping, karyotyping, pathogen-screening, and reanimating ES cell clones generated by the KOMP Mutagenesis Teams and Regeneron, Inc in preparation for distribution to the research community. Services Provided
In addition to obtaining KOMP products (vectors and ES cell clones), investigators will have full access to all UC Davis resources and services, including reanimation (i.e., microinjection) of ES cells into live mice, conversion of ES cells and live mice to cryopreserved embryos and sperm, and conversion of a cryopreserved archive into live mice. The KOMP repository shall also maintain an easily-navigable and informative public website (www.komp.org Contact Information
The Knockout Mouse Project
Website: www.komp.org Grant No.: U42 RR024244
Principal Investigator and Director
Pieter J. de Jong, Ph.D.
Additional Contact: KOMP Manager Mouse Mutant Gene ResourceResearch Emphasis/ObjectivesResearch objectives include the following: to discover and characterize new mouse models of human inherited conditions; to maintain as breeding pairs and preserve as frozen embryos new and established mouse mutations and chromosomal aberrations; to develop genetically suitable stocks of new and established mouse mutations for use in biomedical research; and to make these mutant stocks available to interested investigators in the scientific community. Services Provided
This resource encourages collaborations with visiting investigators to screen mutant and wild-derived strains for specific conditions, symptoms, biochemical or physiological defects, behavior, or other phenotypes of interest. The resource provides technical support for users of JAX mice to answer questions regarding genetics, husbandry, and characteristics of mutant mice. All mice can be ordered by calling The Jackson Laboratory's Customer Service Department at 1-800-422-MICE or 207-288-5845 or by faxing 207-288-6150. A fee for mice is charged to partially recover strain maintenance costs and shipping expenses. For more information about the resource, contact any of the investigators listed below. Updates on strain availability and repository information are accessible at jaxmice.jax.org/findmice/repository.html Available Mouse Strains
This resource develops and maintains strains of mice with specific
mutant genes in various categories, including growth and development,
reproduction, neurological, neuromuscular, vision and hearing,
skeletal, immunological, skin and hair, pigmentation, kidney, and
enzyme deficiencies. It also maintains stocks of mice with chromosomal
aberrations including inversions, translocations, monosomy, and
trisomy. In addition, several wild-derived inbred strains are
maintained for linkage crosses. Updates on strain
availablility and repository information are accessible at jaxmice.jax.org/findmice/repository.html Contact Information
The Jackson Laboratory
Website: www.jax.org/mmr/index.html Grant No.: P40 RR001183
Principal Investigator
Additional Contacts
Cathleen Lutz, Ph.D. Mutant Mouse Regional Resource CentersThe Mutant Mouse Regional Resources Centers (MMRRC) Program consists of centers that collectively operate as a one-stop shop to serve the biomedical research community. The MMRRCs accept, cryopreserve, maintain, and distribute mutant mouse strains for use in biomedical research. The centers take transgenics, knockouts, and all other kinds of induced mutant mouse lines at no cost to the donors, and after specific-pathogen-free rederivation and cryopreservation, distribute mice for a small fee to requesting investigators for noncommercial, academic research purposes only.
The MMRRCs currently have a census of more than 10,000 mutant strains
maintained as either actively breeding mouse colonies, frozen embryos
or germplasm, and/or embryonic stem cell clones. Investigators
interested in donating mouse strains or finding out what mice are
available for distribution should visit: www.mmrrc.org Genetically Engineered Strains Now Available: If you are investigating mutant mouse strains for your research projects, please visit the MMRRC Catalog Search Form on the MMRRC website. The search form provides links to register interest for strains not yet available for distribution and to the Strain (or cell line) Detail Sheets for available mice (and cell lines). Furthermore, the form includes checkboxes that enable researchers to request available strains, strains in development, or strains awaiting transfer from donating investigators, or any combination of the three. Mutant Mouse Regional Resource Center at The Jackson LaboratoryThe Jackson Laboratory (JAX) MMRRC resides within the Genetic Resource Science (GRS) Repository and is managed by GRS personnel with common practices. The GRS Repository program at JAX is comprised of multiple subsets of mice including: spontaneous mutants; mice carrying transgenes, and targeted or chemically induced mutations; panels of consomic and recombinant inbred strains; and disease-specific model strains. The goal of the GRS Repository is to increase access of mouse models to researchers worldwide by efficiently housing and distributing mice, either as live mice or as cryopreserved sperm or embryos, according to the needs of the requesting investigator. Research Emphasis/ObjectivesMutant mice, with phenotypes mimicking human diseases, play a critical role in understanding the mechanisms underlying mammalian biology and human genetic disorders. These valuable resources can only be fully exploited if they are archived, managed and made accessible to the scientific community by centralized repositories. The JAX MMRRC participates with three other MMRRC distribution facilities and the Informatics, Coordination and Service Center (ICSC) to achieve this goal. The objectives of the JAX MMRRC are to: 1) acquire mutant strains; 2) import and archive mutant strains; 3) distribute strains as cryopreserved material, mice recovered from cryopreserved material, ES cell lines or live mice; 4) implement a state-of-the-art quality control program; 5) provide customer support and technical service; 6) promote MMRRC strains; 7) have an interactive repository management plan; 8) establish the bioinformatics infrastructure to operate and to interact with the ICSC and other MMRRC repositories, and 9) conduct research to add utility to JAX MMRRC strains by developing pluripotent stem cell resources from valuable repository disease models using both standard derivation techniques and iPS technology. Current ResearchES cell lines are powerful tools for elucidating disease mechanisms in vitro, and their use is growing dramatically as techniques for their differentiation are refined. However, ES cell lines do not exist for many disease models. Research efforts at the JAX MMRRC are aimed at using standard and inhibitor-mediated derivation techniques, as well as induced pluripotent stem cell technologies, to create additional mouse ES cell resources for the scientific community. The resulting lines will be distributed to the scientific community through the JAX MMRRC. Services ProvidedThe MMRRC at JAX offers the following services in concert with The Jackson Laboratory: breeding and rederivation services including dedicated supply, speed expansion, congenic development, strain rescue, and microinjection; cryopreservation, storage and recovery services including sperm and embryo cryopreservation; phenotyping and pathology services; and genome sciences services, including gene mapping, genome scanning and QTL analysis. Contact Information
JAX MMRRC
Website: www.jax.org/mmrrc/ Grant No.: U42 RR026296
Principal Investigator
Additional Contact Mutant Mouse Regional Resource Center at the University of MissouriThe Mutant Mouse Regional Resource Center (MMRRC) at the University of Missouri (MU-MMRRC) was established in 2000. The MU-MMRRC is a consortium of investigators including John K. Critser, Ph.D.; Beth Bauer, D.V.M; Elizabeth Bryda, Ph.D; Craig Franklin, Ph.D., D.V.M.; and Lela K. Riley, Ph.D., at the University of Missouri. Research Emphasis/ObjectivesResearch projects are focused in the following major areas of importance to mice as animal models: 1) development of efficacious methods for cryopreservation of mouse strains with complex genetics through development of iPS cells; 2) development of methods for high temperature storage of fibroblasts that can later be utilized to establish iPS cells. Services ProvidedThe overall goal is to establish a center for distributing high-quality, well-characterized inbred, hybrid and mutant mice to investigators. To this end, the MU-MMRRC will select and import mouse strains and stocks important to the biomedical research community; rederive mice to a pathogen-free state; cryopreserve gametes and embryos; perform genotyping and infectious disease monitoring to ensure the quality of the mice; and distribute mice to investigators as live mice, cryopreserved germplasm, or tissues. Contact Information
University of Missouri — MMRRC
Website: www.mmrrc.org Grant No.: U42 RR014821
Principal Investigators
Lela K. Riley, Ph.D.
Additional Contact
Customer Service Mutant Mouse Regional Resource Center at University of California, DavisResearch Emphasis/ObjectivesThe overall objective of the UC Davis Mutant Mouse Regional Resource Center is to serve as a component of the MMRRC National Program, to accept, maintain, and distribute mutant mice for use in biomedical research. The MMRRC Program accepts transgenics, knockouts, and all other kinds of mutant mouse lines at no cost to the donor, and after re-derivation and cryopreservation, distributes breeding stock or germplasm of genetically-defined and pathogen-free mice at a small fee to requesting investigators. The UC Davis MMRRC currently has a census of over 10,000 mutant strains that are maintained as live breeding colonies, frozen embryos or germplasm, and embryonic stem cell clones. Furthermore, the MMRRC serves as a center of excellence in mouse biology, mouse genomic manipulation, genetic and genotyping services, strain rescue, pathology and clinical pathology phenotyping, behavioral phenotyping, non-invasive imaging, and infectious disease diagnostics. These services are available to the biomedical research community on a collaborative or fee-for-service basis. The MMRRC provides support for applied research that is aimed at enhancing the resource, and serves as a teaching environment that is linked to residency and graduate training programs in the Center for Comparative Medicine. Current ResearchAreas of applied research that are supported by the MMRRC include evaporative drying of mouse sperm, oocyte and blastocyst cryopreservation, accelerated speed congenics, development of optimized SNPs, multiplex detection of antibody to infectious agents, and vertical transmission of infectious agents through germplasm. Services ProvidedCryopreservation, rederivation, strain rescue, construct design, random and targeted mutations, speed congenics, genotyping, in vitro fertilization, intracytoplasmic sperm injection, pathology and behavioral phenotyping, diagnostics, experimental pathology, non-invasive imaging, data and image analysis, colony management, and teaching resources. Contact Information
Center for Comparative Medicine
Website: ccm.ucdavis.edu Grant No.: U42 RR14905
Principal Investigator Mutant Mouse Regional Resource Center at University of North CarolinaResearch Emphasis/ObjectivesUNC-Chapel Hill is one of four MMRRC centers that are developing and maintaining a mutant mouse resource for the benefit of the entire biomedical community. To meet demands for MMRRC services and improve upon existing technologies, the goals of the UNC-Chapel Hill Center are to: 1) import, archive and distribute genetically modified mouse strains; 2) increase visibility of the MMRRC through advertising and solicitations targeting the biomedical research community; 3) develop improved and reliable methods for assisted fertilization; 4) integrate gnotobiotics technology into MMRRC services; and 5) generate an allelic series of point mutations in genes of interest as a community resource. Current ResearchStreamline and improve operating procedures to increase importation, cryoarchiving and distribution of mice. Develop improved methods for sperm phenotyping, assisted in vitro fertilization, intracytoplasmic sperm injection. Collaborate with the National Gnotobiotic Rodent Resource Center to provide gnotobiotic strains, technology development, and relevant phenotyping services. Generate an allelic series of point mutations in genes of interest. Services ProvidedMaintain selected strains of genetically modified mice; distribute mutant mice from live colony; distribute mutant mice from cryopreserved archive; health testing; frozen sperm, oocytes, ovaries; embryo, cropreservation and rederivation; sperm phenotyping; intracytoplasmic sperm injection; assisted IVF; ES cell library screening for ENU induced point mutations; ES cell microinjection; genotyping; and histopathology. Contact Information
MMRRC-Chapel Hill Grant No.: U42 RR014817
Principal Investigator
Additional Contact MMRRC Informatics, Coordination and Service Center at The Jackson LaboratoryThe Informatics, Coordination and Service Center (ICSC) at The Jackson Laboratory provides centralized processing and infrastructure that allows the separate MMRRC facilities to function as a single resource to the biomedical research community. Research Emphasis/ObjectivesThe MMRRC-ICSC provides a centralized informatics resource and customer service center for the three MMRRC mutant mouse resource centers described above. The ICSC maintains the MMRRC public and internal websites, maintains the database that supports the MMRRC catalog and website, provides software support for MMRRC programmatic projects (e.g., order tracking and customer surveys), provides administrative support for MMRRC monthly meetings and strain review and serves as the customer service center for all mouse strains and ES cell lines held by the MMRRC. Although the ICSC does not carry out research, per se, it is responsible for upgrading and enhancing software and database management systems that support the informatics and customer service activities. Services Provided
The ICSC maintains a website with information on the MMRRC and mouse resources and services available and provides a single contact point for scientists to request mouse strains or ES cell lines held in the four MMRRC resource centers. Mice or ES cells can be ordered by calling the MMRRC-ICSC Customer Service Center at 1-800-910-2291 (North America) or 207-288-6009 (international), by emailing: service@mmrrc.org, or by faxing 207-288-6150. Access the searchable database and online Submission and Order Request Forms at www.mmrrc.org/ Contact Information
MMRRC Informatics, Coordination and Service Center Contract No.: RR62090
Principal Investigator and Contact
Additional Contact National Gnotobiotic Rodent Resource CenterResearch Emphasis/ObjectivesThis resource allows NIH-funded investigators to explore genetic/environmental interactions. Gnotobiotic mice and rats are used to investigate the physiologic and pathophysiologic consequences of colonizing normal and genetically mutated hosts with commensal bacterial flora or with individual components of this complex ecosystem. Contact Information
University of North Carolina-Chapel Hill
Website: www.med.unc.edu/ngrrc Grant No: P40 RR018603
Principal Investigator Peromyscus Genetic Stock CenterResearch Emphasis/ObjectivesDevelopment of cryopreservation techniques that are applicable to Peromyscus sperm to enable long-term preservation of mutant stocks. The mechanism and genetics of hybrid dysgenesis in reciprocal hybrids of P. maniculatus and P. polionotus. Offspring of matings in one direction are markedly oversized; the reciprocal cross produces undersized offspring. Development of an intermediate density map utilizing PCR-based type 1 (functional genes) and type II (microsatellite) markers as well as single gene mutants with coat color or neurological phenotypes; and interspecific crosses between P. maniculatus and P. polionotus as well as whole genome radiation hybrids for linkage determinations. Fruition of the mapping project will benefit a wide range of investigators interested in using Peromyscus to study the genetic basis of speciation, genome imprinting, disease vector biology, and behavioral and physiological adaptation to habitat. Services Provided
The Peromyscus Genetic Stock Center was established in 1985 and
functions to provide a reliable source of genetically defined and
virus-free animals and related materials to the scientific and
educational communities. The center currently keeps nine species and
more than 27 distinctive mutant and other genetically defined stocks.
Included among the species maintained are P. californicus, P.
leucopus, P. eremicus, P. aztecus, P. melanophrys as well as two
subspecies each of P. maniculatus and P. polionotus and
three inbred lines of P. leucopus. Among the mutant stocks are
17 with altered coat phenotypes, 3 with neurological symptoms, and 6
others with developmental/physiological effects. One of these is a
line deficient in alcohol dehydrogenase that has been widely used in
studies of alcohol metabolism. The stock center also supplies
biological materials, including fresh, frozen, and preserved tissues;
molecular probes, and libraries. The center functions as a
clearinghouse for information regarding this genus by sponsoring an
Internet database (i.e., PeroBase Contact Information
University of South Carolina
Website: stkctr.biol.sc.edu Grant No.: P40 RR014279
Principal Investigator
Co-investigator
Contact and Colony Manager Rat Resource and Research CenterThe Rat Resource and Research Center was established in 2001. The center is a multi-institutional consortium of investigators, including John K. Critser, Ph.D.; Beth Bauer, D.V.M.; and Lela K. Riley, Ph.D., at the University of Missouri. Research Emphasis/ObjectivesResearch projects are focused in the following major areas of importance to rats as animal models: 1) development of efficacious methods for nuclear transfer in the rat; 2) development of efficient methods for genome resource banking and strain/stock reconstitution, including ovarian tissue preservation and spermatozoa cryopreservation; 3) improved methods for genotyping; and 4) improved methods for health monitoring of rats using molecular diagnostics and environmental monitoring. Services ProvidedThe overall goal is to establish a center for distributing high-quality, well-characterized inbred, hybrid, and mutant rats to investigators. To this end, the RRRC will select and import rat strains and stocks important to the biomedical research community; rederive rats to a pathogen-free state; cryopreserve gametes and embryos; perform genotyping, phenotyping, and infectious disease monitoring to ensure the quality of the rats; and distribute rats to investigators as live rats, cryopreserved germplasm, or tissues. Contact Information
University of Missouri
Website: www.nrrrc.missouri.edu Grant No.: P40 RR016939
Principal Investigator
Additional Contacts
Beth Bauer, D.V.M. Special Mouse Strains ResourceResearch Emphasis/ObjectivesThe Special Mouse Strains Resource (SMSR) is a resource of special strains of mice that are valuable tools for genetic analysis of complex diseases. They include panels of recombinant inbred and chromosome substitution strains with diverse genetic backgrounds. The mission of the SMSR is to provide these strains of mice to interested investigators and to enhance their value as scientific tools with additional genotyping and phenotyping. Current Research
The SMSR maintains, characterizes and distributes mouse strain panels of recombinant inbred (RI) strains and chromosome substitution (consomic) strains. Holdings as of April 2007 include six RI strain panels and two chromosome substitution panels, one with A/J chromosomes on the C57BL/6J genetic background (made by Joseph Nadeau, Case Western Reserve University) and one with PWD chromosomes on the C57BL/6J background (made by Jiri Forejt in Czechoslovakia). PWD is a pure Mus musculus musculus inbred strain. The Nadeau consomic panel has been characterized for immunological, hematological, behavioral, skeletal, auditory, ocular, and physiological phenotypes to add to baseline information. The Forejt panel is being characterized for many of these same phenotypes. All of these data will be made publicly available online in the Phenome database Services Provided
The resource provides technical support for users of JAX mice to
answer questions regarding genetics, husbandry, and characteristics of
mutant mice. All mice can be ordered by calling The Jackson
Laboratory's Customer Service Department at 1-800-422-MICE or
207-288-5845 or by faxing 207-288-6150. Access the searchable database Contact Information
The Jackson Laboratory
Website: www.jax.org Grant No.: P40 RR16049
Principal Investigator
Additional Contacts
Cathleen Lutz, Ph.D. |
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National Institutes of Health (NIH) Bethesda, Maryland 20892 NIH . . . Turning Discovery Into Health |
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Department of Health and Human Services |
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